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cytof 2 mass cytometer  (fluidigm)


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    Structured Review

    fluidigm cytof 2 mass cytometer
    Cytof 2 Mass Cytometer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytof+2+mass+cytometer/CyTOF+XT/pmc09443461__ADVS___9___2201409___s001-138-41-46
    Average 93 stars, based on 605 article reviews
    cytof 2 mass cytometer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Suspension:

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The following metal-tagged antibodies purchased from Fluidigm were used for mass cytometry analysis: 89Y-anti-CD45, 175Lu-anti-CD4, 141Pr-anti–PD-1, 143Nd-anti-CD11b, 144Nd-anti-Siglec F, 145Nd-anti-CD69, 146Nd-anti-CD206, 148Nd-anti-Tbet, 114Sn-anti-CD103, 151Eu-anti-CD68, 152Gd-anti-CD3e, 156Gd-anti-CD14, 159Tb-anti-F4/80, 160Dy-anti-CD62L, 161Dy-anti-Ki67, 162Dy-anti-Ly-6C, 165Ho-anti-Foxp3, 149Sm-anti-CD19, 167Er-anti-GATA3, 142Ce-anti-NK1.1, 168Er-anti-CD8a, 172Yb-anti-CD86, 173Yb-anti-CD117, 174Yb-anti-ly-6G/Ly-6C(Gr-1), 209Bi-anti-I-A/I-E, 150Sm-anti-CD11c, and 169Tm-CTLA4. .. The cell suspension was diluted to a concentration of approximately 10 6 cells per mL using double-distilled water containing bead standards and analyzed using a CyTOF 2 mass cytometer from Fluidigm. .. Data normalization and manual gating were performed using the cloud-based Cytobank data analysis platform (RRID: SCR_014043).

    Concentration Assay:

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The following metal-tagged antibodies purchased from Fluidigm were used for mass cytometry analysis: 89Y-anti-CD45, 175Lu-anti-CD4, 141Pr-anti–PD-1, 143Nd-anti-CD11b, 144Nd-anti-Siglec F, 145Nd-anti-CD69, 146Nd-anti-CD206, 148Nd-anti-Tbet, 114Sn-anti-CD103, 151Eu-anti-CD68, 152Gd-anti-CD3e, 156Gd-anti-CD14, 159Tb-anti-F4/80, 160Dy-anti-CD62L, 161Dy-anti-Ki67, 162Dy-anti-Ly-6C, 165Ho-anti-Foxp3, 149Sm-anti-CD19, 167Er-anti-GATA3, 142Ce-anti-NK1.1, 168Er-anti-CD8a, 172Yb-anti-CD86, 173Yb-anti-CD117, 174Yb-anti-ly-6G/Ly-6C(Gr-1), 209Bi-anti-I-A/I-E, 150Sm-anti-CD11c, and 169Tm-CTLA4. .. The cell suspension was diluted to a concentration of approximately 10 6 cells per mL using double-distilled water containing bead standards and analyzed using a CyTOF 2 mass cytometer from Fluidigm. .. Data normalization and manual gating were performed using the cloud-based Cytobank data analysis platform (RRID: SCR_014043).

    Cytometry:

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The following metal-tagged antibodies purchased from Fluidigm were used for mass cytometry analysis: 89Y-anti-CD45, 175Lu-anti-CD4, 141Pr-anti–PD-1, 143Nd-anti-CD11b, 144Nd-anti-Siglec F, 145Nd-anti-CD69, 146Nd-anti-CD206, 148Nd-anti-Tbet, 114Sn-anti-CD103, 151Eu-anti-CD68, 152Gd-anti-CD3e, 156Gd-anti-CD14, 159Tb-anti-F4/80, 160Dy-anti-CD62L, 161Dy-anti-Ki67, 162Dy-anti-Ly-6C, 165Ho-anti-Foxp3, 149Sm-anti-CD19, 167Er-anti-GATA3, 142Ce-anti-NK1.1, 168Er-anti-CD8a, 172Yb-anti-CD86, 173Yb-anti-CD117, 174Yb-anti-ly-6G/Ly-6C(Gr-1), 209Bi-anti-I-A/I-E, 150Sm-anti-CD11c, and 169Tm-CTLA4. .. The cell suspension was diluted to a concentration of approximately 10 6 cells per mL using double-distilled water containing bead standards and analyzed using a CyTOF 2 mass cytometer from Fluidigm. .. Data normalization and manual gating were performed using the cloud-based Cytobank data analysis platform (RRID: SCR_014043).

    Article Title: Human autoimmunity at single cell resolution in aplastic anemia before and after effective immunotherapy
    Article Snippet: Subsequently, intracellular staining for transcription factors was performed after fixation and permeabilization according to the manufacturer’s instructions (eBioscience). .. The CyTOF-2 mass cytometer (Fluidigm) was used for data acquisition. ..

    Article Title: Human autoimmunity at single cell resolution in aplastic anemia before and after effective immunotherapy.
    Article Snippet: The CyTOF-2 mass cytometer (Fluidigm) was used for data acquisition. .. The CyTOF-2 mass cytometer (Fluidigm) was used for data acquisition. ..

    Article Title: Therapeutic radiation drives leptomeningeal dissemination of medulloblastoma through an innate immune process.
    Article Snippet: In brief Radiation, the most effective treatment for medulloblastoma, induces an innate immune response and opens the bloodbrain barrier, allowing for the intravasation of tumor cells into the blood circulation.. These circulating tumor cells can seed the leptomeninges, forming metastasis, the major cause of mortality in patients, whereas inhibition of inflammation abrogates radiationinduced metastasis.

    Article Title: Supporting Information
    Article Snippet: .. The sample was prepared (3 x 10 6 cells/ 50 L Maxpar Cell Staining Buffer) for the staining step as described in the protocol of Maxpar Mouse Sp/LN Phenotyping Panel Kit (Fluidigm, South San Francisco, CA, USA) and performed using CyTOF 2 Mass Cytometer (Fluidigm, South San Francisco CA, USA) at the Genomics Research Center, Academia Sinica). .. CyTOF data were uploaded and analyzed using Cytobank (Beckman Coulter Life Sciences, Pasadena, CA, USA), and gating strategy was applied to identify cell populations was used for making the viSNE plots.

    Article Title: High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.
    Article Snippet: Each well was resuspended in 150 μl of CSM-T and analysed by a CytoFlex flow cytometer (Beckman Coulter). .. The beads were analysed using a CyTOF 2 mass cytometer (Fluidigm), at a rate of 500 events per second. ..

    Article Title: High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.
    Article Snippet: Then, 100 μl of diluted spike S1 protein (2 ng μl−1) in CSM-T (Sino Biological, number 40591-V02H) was added to each well and incubated for 10 min at room temperature. .. The beads were analysed using a CyTOF 2 mass cytometer (Fluidigm) at a rate of 500 events per second. ..

    other:

    Article Title: IL-6 underlies microenvironment immunosuppression and resistance to therapy in glioblastoma
    Article Snippet: Cells were washed in cell-staining media then incubated with 1mL PBS containing 1:5000 Iridium intercalator (Fluidigm) and 4% final formaldehyde.

    Mass Cytometry:

    Article Title: Therapeutic radiation drives leptomeningeal dissemination of medulloblastoma through an innate immune process.
    Article Snippet: In brief Radiation, the most effective treatment for medulloblastoma, induces an innate immune response and opens the bloodbrain barrier, allowing for the intravasation of tumor cells into the blood circulation.. These circulating tumor cells can seed the leptomeninges, forming metastasis, the major cause of mortality in patients, whereas inhibition of inflammation abrogates radiationinduced metastasis.

    Staining:

    Article Title: Supporting Information
    Article Snippet: .. The sample was prepared (3 x 10 6 cells/ 50 L Maxpar Cell Staining Buffer) for the staining step as described in the protocol of Maxpar Mouse Sp/LN Phenotyping Panel Kit (Fluidigm, South San Francisco, CA, USA) and performed using CyTOF 2 Mass Cytometer (Fluidigm, South San Francisco CA, USA) at the Genomics Research Center, Academia Sinica). .. CyTOF data were uploaded and analyzed using Cytobank (Beckman Coulter Life Sciences, Pasadena, CA, USA), and gating strategy was applied to identify cell populations was used for making the viSNE plots.



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